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High-throughput in vitro processing of human primary microRNA by the recombinant microprocessor
We describe a protocol to conduct a high-throughput in vitro processing assay, using 1,881 human primary microRNAs (pri-miRNAs) and recombinant Microprocessor complex, followed by deep sequencing library generation. This comprehensive approach allows the mapping of cleavage sites and the measurement...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749294/ https://www.ncbi.nlm.nih.gov/pubmed/35036952 http://dx.doi.org/10.1016/j.xpro.2021.101042 |