Protocol for reading and imaging live-cell PKA activity using ExRai-AKAR2

Fluorescent protein (FP)-based kinase activity biosensors are powerful tools for probing the spatiotemporal dynamics of signaling pathways in living cells. Yet, the limited sensitivity of most kinase biosensors restricts their reliable application in high-throughput detection modalities. Here, we re...

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Detalles Bibliográficos
Autores principales: Zhang, Jin-fan, Mehta, Sohum, Zhang, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749300/
https://www.ncbi.nlm.nih.gov/pubmed/35036953
http://dx.doi.org/10.1016/j.xpro.2021.101071

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749300/
https://www.ncbi.nlm.nih.gov/pubmed/35036953
http://dx.doi.org/10.1016/j.xpro.2021.101071

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