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Protocol for reading and imaging live-cell PKA activity using ExRai-AKAR2
Fluorescent protein (FP)-based kinase activity biosensors are powerful tools for probing the spatiotemporal dynamics of signaling pathways in living cells. Yet, the limited sensitivity of most kinase biosensors restricts their reliable application in high-throughput detection modalities. Here, we re...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749300/ https://www.ncbi.nlm.nih.gov/pubmed/35036953 http://dx.doi.org/10.1016/j.xpro.2021.101071 |