Effects of Dietary Organic and Inorganic Sulfur on Laying Performance, Egg Quality, Ileal Morphology, and Antioxidant Capacity in Laying Hens

SIMPLE SUMMARY: Oxidative stress caused by environmental and nutritional factors could be detrimental to poultry production. Dietary natural antioxidants could therefore be beneficial in limiting the deleterious effects of oxidative stress in chickens. Methyl sulfonyl methane is a non-toxic natural organosulfur compound with the chemical formula (CH(3))(2)SO(2) and is known as methyl sulfone or dimethyl sulfone. Inorganic sulfate (e.g., sodium sulfate) is involved in the metabolism of many tissues and systems, as well as in important detoxication mechanisms. Dietary sulfur in either organic or inorganic forms exhibits beneficial antioxidant properties in various animals in vivo and in vitro. Therefore, our studies have been conducted to evaluate the role of organic and inorganic sulfur in laying hens. ABSTRACT: The present study was conducted to investigate the comparative effects of organic and inorganic forms of sulfur, methyl sulfonyl methane (MSM) and sodium sulfate (SS), on laying performance, egg quality, ileal morphology, ileal volatile fatty acids, and antioxidant and stress markers in various biological samples in aged laying hens. A total of 144, 73-week-old Lohman Brown-Lite laying hens were randomly assigned to one of three experimental diets: basal diet (CONT), CONT + 0.2% MSM (MSM), and CONT + 0.3% SS (SS). The trial lasted for 12 weeks. MSM and SS diets contained 0.07% of sulfur, either organic or inorganic. Dietary MSM did not affect egg production or feed conversion ratio at 12 weeks compared with the CONT group. Dietary sulfur did not affect egg quality except for the Haugh unit at 4 weeks, which was lowered (p < 0.05) in the SS group. Compared with the CONT group, a higher (p < 0.05) villus height to crypt depth ratio was observed in the SS group. Dietary sulfur did not affect the percentages of short-chain fatty acids in the ileum. Total antioxidant capacity of the liver increased (p < 0.05) in laying hens fed MSM- and SS-added diets compared with the CONT group. The MSM and SS groups were found to have lowered (p < 0.05) malondialdehyde (MDA) concentration in serum samples compared with CONT. Finally, dietary MSM had the lowest (p < 0.05) MDA concentrations in yolk samples. Taken together, our study showed that dietary organic and inorganic sulfur have positive effects on ileal morphology and antioxidant capacity in laying hens. However, SS-mediated inhibition in laying performance needs to be clarified.