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Automated High-Throughput Method for the Fast, Robust, and Reproducible Enrichment of Newly Synthesized Proteins

[Image: see text] A high-throughput method was developed for the automated enrichment of newly synthesized proteins (NSPs), which are labeled metabolically by substituting methionine with the “click-able” analogue azidohomoalanine (AHA). A suitable conjugate containing a dibenzocyclooctyne (DBCO) gr...

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Detalles Bibliográficos
Autores principales: Vargas-Diaz, David, Altelaar, Maarten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749957/
https://www.ncbi.nlm.nih.gov/pubmed/34860524
http://dx.doi.org/10.1021/acs.jproteome.1c00743