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Automated High-Throughput Method for the Fast, Robust, and Reproducible Enrichment of Newly Synthesized Proteins
[Image: see text] A high-throughput method was developed for the automated enrichment of newly synthesized proteins (NSPs), which are labeled metabolically by substituting methionine with the “click-able” analogue azidohomoalanine (AHA). A suitable conjugate containing a dibenzocyclooctyne (DBCO) gr...
Autores principales: | Vargas-Diaz, David, Altelaar, Maarten |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8749957/ https://www.ncbi.nlm.nih.gov/pubmed/34860524 http://dx.doi.org/10.1021/acs.jproteome.1c00743 |
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