Detection and analysis of long noncoding RNA expression profiles related to epithelial–mesenchymal transition in keloids

BACKGROUND: The role of epithelial-mesenchymal transition (EMT) in the pathogenesis of keloids is currently raising increasing attention. Long noncoding RNAs (lncRNAs) govern a variety of biological processes, such as EMT, and their dysregulation is involved in many diseases including keloid disease...

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Autores principales: Chen, Zhixiong, Chu, Xi, Xu, Jinghong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751032/
https://www.ncbi.nlm.nih.gov/pubmed/35012558
http://dx.doi.org/10.1186/s12938-022-00976-x
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author Chen, Zhixiong
Chu, Xi
Xu, Jinghong
author_facet Chen, Zhixiong
Chu, Xi
Xu, Jinghong
author_sort Chen, Zhixiong
collection PubMed
description BACKGROUND: The role of epithelial-mesenchymal transition (EMT) in the pathogenesis of keloids is currently raising increasing attention. Long noncoding RNAs (lncRNAs) govern a variety of biological processes, such as EMT, and their dysregulation is involved in many diseases including keloid disease. The aim of this study was to identify differentially expressed EMT-related lncRNAs in keloid tissues versus normal tissues and to interpret their functions. RESULTS: Eleven lncRNAs and 16 mRNAs associated with EMT were identified to have differential expression between keloid and normal skin tissues (fold change > 1.5, P < 0.05). Gene Ontology (GO) analysis showed that these differentially expressed mRNAs functioned in the extracellular matrix, protein binding, the positive regulation of cellular processes, the Set1C/COMPASS complex and histone acetyltransferase activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that these mRNAs are involved in pathways in cancer. The lncRNA, XLOC_000587 may promote cell proliferation and migration by enhancing the expression of ENAH, while AF268386 may facilitate the invasive growth of keloids by upregulating DDR2. CONCLUSIONS: We characterized the differential expression profiles of EMT-related lncRNAs and mRNAs in keloids, which may contribute to preventing the occurrence and development of keloids by targeting the corresponding signaling pathways. These lncRNAs and mRNAs may provide biomarkers for keloid diagnosis and serve as potential targets for the treatment of this disease. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12938-022-00976-x.
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spelling pubmed-87510322022-01-11 Detection and analysis of long noncoding RNA expression profiles related to epithelial–mesenchymal transition in keloids Chen, Zhixiong Chu, Xi Xu, Jinghong Biomed Eng Online Research BACKGROUND: The role of epithelial-mesenchymal transition (EMT) in the pathogenesis of keloids is currently raising increasing attention. Long noncoding RNAs (lncRNAs) govern a variety of biological processes, such as EMT, and their dysregulation is involved in many diseases including keloid disease. The aim of this study was to identify differentially expressed EMT-related lncRNAs in keloid tissues versus normal tissues and to interpret their functions. RESULTS: Eleven lncRNAs and 16 mRNAs associated with EMT were identified to have differential expression between keloid and normal skin tissues (fold change > 1.5, P < 0.05). Gene Ontology (GO) analysis showed that these differentially expressed mRNAs functioned in the extracellular matrix, protein binding, the positive regulation of cellular processes, the Set1C/COMPASS complex and histone acetyltransferase activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis demonstrated that these mRNAs are involved in pathways in cancer. The lncRNA, XLOC_000587 may promote cell proliferation and migration by enhancing the expression of ENAH, while AF268386 may facilitate the invasive growth of keloids by upregulating DDR2. CONCLUSIONS: We characterized the differential expression profiles of EMT-related lncRNAs and mRNAs in keloids, which may contribute to preventing the occurrence and development of keloids by targeting the corresponding signaling pathways. These lncRNAs and mRNAs may provide biomarkers for keloid diagnosis and serve as potential targets for the treatment of this disease. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12938-022-00976-x. BioMed Central 2022-01-11 /pmc/articles/PMC8751032/ /pubmed/35012558 http://dx.doi.org/10.1186/s12938-022-00976-x Text en © The Author(s) 2022, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Chen, Zhixiong
Chu, Xi
Xu, Jinghong
Detection and analysis of long noncoding RNA expression profiles related to epithelial–mesenchymal transition in keloids
title Detection and analysis of long noncoding RNA expression profiles related to epithelial–mesenchymal transition in keloids
title_full Detection and analysis of long noncoding RNA expression profiles related to epithelial–mesenchymal transition in keloids
title_fullStr Detection and analysis of long noncoding RNA expression profiles related to epithelial–mesenchymal transition in keloids
title_full_unstemmed Detection and analysis of long noncoding RNA expression profiles related to epithelial–mesenchymal transition in keloids
title_short Detection and analysis of long noncoding RNA expression profiles related to epithelial–mesenchymal transition in keloids
title_sort detection and analysis of long noncoding rna expression profiles related to epithelial–mesenchymal transition in keloids
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8751032/
https://www.ncbi.nlm.nih.gov/pubmed/35012558
http://dx.doi.org/10.1186/s12938-022-00976-x
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