Protocol for identification and validation of 2′3′-cGAMP-binding proteins by photoaffinity probes

Mammalian cyclic dinucleotide 2′3′-cGAMP functions as a second messenger in innate immune response. Here, we report a protocol to utilize 2′3′-cGAMP photoaffinity probes to capture 2′3′-cGAMP-binding or 2′3′-cGAMP-interacting proteins from HeLa cell lysate for in-gel visualization by fluorescent ima...

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Detalles Bibliográficos
Autores principales: Hou, Yingjie, Lu, Heng, Sun, Le, Zhang, Yaoyang, Jiang, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8752945/
https://www.ncbi.nlm.nih.gov/pubmed/35036957
http://dx.doi.org/10.1016/j.xpro.2021.101076

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8752945/
https://www.ncbi.nlm.nih.gov/pubmed/35036957
http://dx.doi.org/10.1016/j.xpro.2021.101076

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