Cargando…
Double nicking by RNA-directed Cascade-nCas3 for high-efficiency large-scale genome engineering
New CRISPR-based genome editing technologies are developed to continually drive advances in life sciences, which, however, are predominantly derived from systems of Type II CRISPR-Cas9 and Type V CRISPR-Cas12a for eukaryotes. Here we report a novel CRISPR-n(nickase)Cas3 genome editing tool establish...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8753164/ https://www.ncbi.nlm.nih.gov/pubmed/35016549 http://dx.doi.org/10.1098/rsob.210241 |