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Double nicking by RNA-directed Cascade-nCas3 for high-efficiency large-scale genome engineering

New CRISPR-based genome editing technologies are developed to continually drive advances in life sciences, which, however, are predominantly derived from systems of Type II CRISPR-Cas9 and Type V CRISPR-Cas12a for eukaryotes. Here we report a novel CRISPR-n(nickase)Cas3 genome editing tool establish...

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Detalles Bibliográficos
Autores principales:	Hao, Yile, Wang, Qinhua, Li, Jie, Yang, Shihui, Zheng, Yanli, Peng, Wenfang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Royal Society 2022
Materias:	Research
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8753164/ https://www.ncbi.nlm.nih.gov/pubmed/35016549 http://dx.doi.org/10.1098/rsob.210241

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