Chemical capping improves template switching and enhances sequencing of small RNAs

Template-switching reverse transcription is widely used in RNA sequencing for low-input and low-quality samples, including RNA from single cells or formalin-fixed paraffin-embedded (FFPE) tissues. Previously, we identified the native eukaryotic mRNA 5′ cap as a key structural element for enhancing t...

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Detalles Bibliográficos
Autores principales: Wulf, Madalee G, Maguire, Sean, Dai, Nan, Blondel, Alice, Posfai, Dora, Krishnan, Keerthana, Sun, Zhiyi, Guan, Shengxi, Corrêa, Ivan R
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8754658/
https://www.ncbi.nlm.nih.gov/pubmed/34581823
http://dx.doi.org/10.1093/nar/gkab861

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8754658/
https://www.ncbi.nlm.nih.gov/pubmed/34581823
http://dx.doi.org/10.1093/nar/gkab861

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