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Chemical capping improves template switching and enhances sequencing of small RNAs

Template-switching reverse transcription is widely used in RNA sequencing for low-input and low-quality samples, including RNA from single cells or formalin-fixed paraffin-embedded (FFPE) tissues. Previously, we identified the native eukaryotic mRNA 5′ cap as a key structural element for enhancing t...

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Detalles Bibliográficos
Autores principales:	Wulf, Madalee G, Maguire, Sean, Dai, Nan, Blondel, Alice, Posfai, Dora, Krishnan, Keerthana, Sun, Zhiyi, Guan, Shengxi, Corrêa, Ivan R
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8754658/ https://www.ncbi.nlm.nih.gov/pubmed/34581823 http://dx.doi.org/10.1093/nar/gkab861

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