Ethylenediamine derivatives efficiently react with oxidized RNA 3′ ends providing access to mono and dually labelled RNA probes for enzymatic assays and in vivo translation

Development of RNA-based technologies relies on the ability to detect, manipulate, and modify RNA. Efficient, selective and scalable covalent modification of long RNA molecules remains a challenge. We report a chemical method for modification of RNA 3′-end based on previously unrecognized superior r...

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Detalles Bibliográficos
Autores principales: Mamot, Adam, Sikorski, Pawel J, Siekierska, Aleksandra, de Witte, Peter, Kowalska, Joanna, Jemielity, Jacek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755103/
https://www.ncbi.nlm.nih.gov/pubmed/34591964
http://dx.doi.org/10.1093/nar/gkab867

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755103/
https://www.ncbi.nlm.nih.gov/pubmed/34591964
http://dx.doi.org/10.1093/nar/gkab867

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