CRISPitope: A generic platform to model target antigens for adoptive T cell transfer therapy in mouse tumor models

This protocol details the procedure for CRISPR-assisted insertion of epitopes (CRISPitope), a flexible approach for generating tumor cells expressing model CD8(+) T cell epitopes fused to endogenously encoded gene products of choice. CRISPitope-engineered tumor cells can be recognized by T cell rece...

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Detalles Bibliográficos
Autores principales: Effern, Maike, Glodde, Nicole, Bawden, Emma, Liebing, Jana, Hinze, Daniel, Tüting, Thomas, Gebhardt, Thomas, Hölzel, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755566/
https://www.ncbi.nlm.nih.gov/pubmed/35059651
http://dx.doi.org/10.1016/j.xpro.2021.101038

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755566/
https://www.ncbi.nlm.nih.gov/pubmed/35059651
http://dx.doi.org/10.1016/j.xpro.2021.101038

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