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CRISPitope: A generic platform to model target antigens for adoptive T cell transfer therapy in mouse tumor models
This protocol details the procedure for CRISPR-assisted insertion of epitopes (CRISPitope), a flexible approach for generating tumor cells expressing model CD8(+) T cell epitopes fused to endogenously encoded gene products of choice. CRISPitope-engineered tumor cells can be recognized by T cell rece...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8755566/ https://www.ncbi.nlm.nih.gov/pubmed/35059651 http://dx.doi.org/10.1016/j.xpro.2021.101038 |