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Comparative analysis of DNA extraction and PCR product purification methods for cervicovaginal microbiome analysis using cpn60 microbial profiling

BACKGROUND: The microbiota of the lower female genital tract plays an important role in women’s health. Microbial profiling using the chaperonin60 (cpn60) universal target (UT) improves resolution of vaginal species associated with negative health outcomes compared to the more commonly used 16S ribo...

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Detalles Bibliográficos
Autores principales: Shvartsman, Elinor, Richmond, Meika E. I., Schellenberg, John J., Lamont, Alana, Perciani, Catia, Russell, Justen N. H., Poliquin, Vanessa, Burgener, Adam, Jaoko, Walter, Sandstrom, Paul, MacDonald, Kelly S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758110/
https://www.ncbi.nlm.nih.gov/pubmed/35025956
http://dx.doi.org/10.1371/journal.pone.0262355