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Comparative analysis of DNA extraction and PCR product purification methods for cervicovaginal microbiome analysis using cpn60 microbial profiling
BACKGROUND: The microbiota of the lower female genital tract plays an important role in women’s health. Microbial profiling using the chaperonin60 (cpn60) universal target (UT) improves resolution of vaginal species associated with negative health outcomes compared to the more commonly used 16S ribo...
Autores principales: | Shvartsman, Elinor, Richmond, Meika E. I., Schellenberg, John J., Lamont, Alana, Perciani, Catia, Russell, Justen N. H., Poliquin, Vanessa, Burgener, Adam, Jaoko, Walter, Sandstrom, Paul, MacDonald, Kelly S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8758110/ https://www.ncbi.nlm.nih.gov/pubmed/35025956 http://dx.doi.org/10.1371/journal.pone.0262355 |
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