Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium

A series of Bifidobacterium-Escherichia coli shuttle vectors (pKO403-lacZ′-Cm, pKO403-lacZ′-Sp, pKO403-lacZ′-p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the lacZ′α fragment, overhung by two facing type IIS restric...

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Detalles Bibliográficos
Autores principales: Altaib, Hend, Ozaki, Yuka, Kozakai, Tomoya, Sakaguchi, Kouta, Nomura, Izumi, Suzuki, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Other Genetic Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759359/
https://www.ncbi.nlm.nih.gov/pubmed/35023783
http://dx.doi.org/10.1128/mra.00884-21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759359/
https://www.ncbi.nlm.nih.gov/pubmed/35023783
http://dx.doi.org/10.1128/mra.00884-21

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