Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium

A series of Bifidobacterium-Escherichia coli shuttle vectors (pKO403-lacZ′-Cm, pKO403-lacZ′-Sp, pKO403-lacZ′-p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the lacZ′α fragment, overhung by two facing type IIS restric...

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Detalles Bibliográficos
Autores principales: Altaib, Hend, Ozaki, Yuka, Kozakai, Tomoya, Sakaguchi, Kouta, Nomura, Izumi, Suzuki, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Other Genetic Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759359/
https://www.ncbi.nlm.nih.gov/pubmed/35023783
http://dx.doi.org/10.1128/mra.00884-21
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author Altaib, Hend
Ozaki, Yuka
Kozakai, Tomoya
Sakaguchi, Kouta
Nomura, Izumi
Suzuki, Tohru
author_facet Altaib, Hend
Ozaki, Yuka
Kozakai, Tomoya
Sakaguchi, Kouta
Nomura, Izumi
Suzuki, Tohru
author_sort Altaib, Hend
collection PubMed
description A series of Bifidobacterium-Escherichia coli shuttle vectors (pKO403-lacZ′-Cm, pKO403-lacZ′-Sp, pKO403-lacZ′-p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the lacZ′α fragment, overhung by two facing type IIS restriction sites, for blue-white selection and seamless gene cloning. These vectors are useful for gene knockout or multigene integration into the chromosome of Bifidobacterium.
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spelling pubmed-87593592022-01-24 Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium Altaib, Hend Ozaki, Yuka Kozakai, Tomoya Sakaguchi, Kouta Nomura, Izumi Suzuki, Tohru Microbiol Resour Announc Other Genetic Resources A series of Bifidobacterium-Escherichia coli shuttle vectors (pKO403-lacZ′-Cm, pKO403-lacZ′-Sp, pKO403-lacZ′-p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the lacZ′α fragment, overhung by two facing type IIS restriction sites, for blue-white selection and seamless gene cloning. These vectors are useful for gene knockout or multigene integration into the chromosome of Bifidobacterium. American Society for Microbiology 2022-01-13 /pmc/articles/PMC8759359/ /pubmed/35023783 http://dx.doi.org/10.1128/mra.00884-21 Text en Copyright © 2022 Altaib et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Other Genetic Resources
Altaib, Hend
Ozaki, Yuka
Kozakai, Tomoya
Sakaguchi, Kouta
Nomura, Izumi
Suzuki, Tohru
Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium
title Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium
title_full Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium
title_fullStr Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium
title_full_unstemmed Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium
title_short Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium
title_sort bifidobacterium-escherichia coli shuttle vector series pko403, with temperature-sensitive replication origin for gene knockout in bifidobacterium
topic Other Genetic Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759359/
https://www.ncbi.nlm.nih.gov/pubmed/35023783
http://dx.doi.org/10.1128/mra.00884-21
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