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Bifidobacterium-Escherichia coli Shuttle Vector Series pKO403, with Temperature-Sensitive Replication Origin for Gene Knockout in Bifidobacterium
A series of Bifidobacterium-Escherichia coli shuttle vectors (pKO403-lacZ′-Cm, pKO403-lacZ′-Sp, pKO403-lacZ′-p15A) were constructed based on the pKO403 backbone, which carries a temperature-sensitive replication origin. These vectors carry the lacZ′α fragment, overhung by two facing type IIS restric...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society for Microbiology
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8759359/ https://www.ncbi.nlm.nih.gov/pubmed/35023783 http://dx.doi.org/10.1128/mra.00884-21 |