A kinetic intra-cellular assay (KICA) to measure quantitative compound binding kinetics within living cells

The Kinetic Intra-Cellular Assay (KICA) is a recombinant cell-based technique that utilizes NanoBRET technology. KICA enables the measurement of intracellular binding kinetics. This protocol describes steps for cellular transfection and expression, followed by addition of a target specific fluoropho...

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Detalles Bibliográficos
Autores principales: Lay, Charles S., Thomas, Daniel A., Evans, John P., Jones, Emma J., Gatfield, Kelly M., Craggs, Peter D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760444/
https://www.ncbi.nlm.nih.gov/pubmed/35059653
http://dx.doi.org/10.1016/j.xpro.2021.101078

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760444/
https://www.ncbi.nlm.nih.gov/pubmed/35059653
http://dx.doi.org/10.1016/j.xpro.2021.101078

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