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A kinetic intra-cellular assay (KICA) to measure quantitative compound binding kinetics within living cells
The Kinetic Intra-Cellular Assay (KICA) is a recombinant cell-based technique that utilizes NanoBRET technology. KICA enables the measurement of intracellular binding kinetics. This protocol describes steps for cellular transfection and expression, followed by addition of a target specific fluoropho...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760444/ https://www.ncbi.nlm.nih.gov/pubmed/35059653 http://dx.doi.org/10.1016/j.xpro.2021.101078 |