Protocol for single-cell isolation and genome amplification of environmental microbial eukaryotes for genomic analysis

We describe environmental microbial eukaryotes (EMEs) sample collection, single-cell isolation, lysis, and genome amplification, followed by the rDNA amplification and OTU screening for recovery of high-quality species-specific genomes for de novo assembly. These protocols are part of our pipeline t...

Descripción completa

Detalles Bibliográficos
Autores principales: Ciobanu, Doina, Chan, Sandy, Ahrendt, Steven, Quandt, C. Alisha, Benny, Gerald L., Smith, Matthew E., James, Timothy Y., Cheng, Jan-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760549/
https://www.ncbi.nlm.nih.gov/pubmed/35059648
http://dx.doi.org/10.1016/j.xpro.2021.100968

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760549/
https://www.ncbi.nlm.nih.gov/pubmed/35059648
http://dx.doi.org/10.1016/j.xpro.2021.100968

Ejemplares similares