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Protocol for single-cell isolation and genome amplification of environmental microbial eukaryotes for genomic analysis
We describe environmental microbial eukaryotes (EMEs) sample collection, single-cell isolation, lysis, and genome amplification, followed by the rDNA amplification and OTU screening for recovery of high-quality species-specific genomes for de novo assembly. These protocols are part of our pipeline t...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8760549/ https://www.ncbi.nlm.nih.gov/pubmed/35059648 http://dx.doi.org/10.1016/j.xpro.2021.100968 |