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Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction
We aimed to describe the SARS-CoV-2 lineages circulating early pandemic among samples with S gene dropout and characterize the receptor-binding domain (RBD) of viral spike protein. Adults and children older than 2 months with signs and symptoms of COVID-19 were prospectively enrolled from May to Oct...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761118/ https://www.ncbi.nlm.nih.gov/pubmed/35219552 http://dx.doi.org/10.1016/j.diagmicrobio.2022.115636 |
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author | Sartor, Ivaine Tais Sauthier Varela, Fernanda Hammes Meireles, Mariana Rost Kern, Luciane Beatriz Azevedo, Thaís Raupp Giannini, Gabriela Luchiari Tumioto da Silva, Mariana Soares Demoliner, Meriane Gularte, Juliana Schons de Almeida, Paula Rodrigues Fleck, Juliane Deise Zavaglia, Gabriela Oliveira Fernandes, Ingrid Rodrigues de David, Caroline Nespolo Santos, Amanda Paz de Almeida, Walquiria Aparecida Ferreira Porto, Victor Bertollo Gomes Scotta, Marcelo Comerlato Vieira, Gustavo Fioravanti Spilki, Fernando Rosado Stein, Renato T. Polese-Bonatto, Márcia |
author_facet | Sartor, Ivaine Tais Sauthier Varela, Fernanda Hammes Meireles, Mariana Rost Kern, Luciane Beatriz Azevedo, Thaís Raupp Giannini, Gabriela Luchiari Tumioto da Silva, Mariana Soares Demoliner, Meriane Gularte, Juliana Schons de Almeida, Paula Rodrigues Fleck, Juliane Deise Zavaglia, Gabriela Oliveira Fernandes, Ingrid Rodrigues de David, Caroline Nespolo Santos, Amanda Paz de Almeida, Walquiria Aparecida Ferreira Porto, Victor Bertollo Gomes Scotta, Marcelo Comerlato Vieira, Gustavo Fioravanti Spilki, Fernando Rosado Stein, Renato T. Polese-Bonatto, Márcia |
author_sort | Sartor, Ivaine Tais Sauthier |
collection | PubMed |
description | We aimed to describe the SARS-CoV-2 lineages circulating early pandemic among samples with S gene dropout and characterize the receptor-binding domain (RBD) of viral spike protein. Adults and children older than 2 months with signs and symptoms of COVID-19 were prospectively enrolled from May to October in Porto Alegre, Brazil. All participants performed RT-PCR assay, and samples with S gene dropout and cycle threshold < 30 were submitted to high-throughput sequencing (HTS). 484 out of 1,557 participants tested positive for SARS-CoV-2. The S gene dropout was detected in 7.4% (36/484) and a peak was observed in August. The B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic. The RBD novel mutation (Y380Q) was found in one sample occurring simultaneously with C379W and V395A, and the B.1.91 lineage in the spike protein. The Y380Q and C379W may interfere with the binding of neutralizing antibodies (CR3022, EY6A, H014, S304). |
format | Online Article Text |
id | pubmed-8761118 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87611182022-01-18 Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction Sartor, Ivaine Tais Sauthier Varela, Fernanda Hammes Meireles, Mariana Rost Kern, Luciane Beatriz Azevedo, Thaís Raupp Giannini, Gabriela Luchiari Tumioto da Silva, Mariana Soares Demoliner, Meriane Gularte, Juliana Schons de Almeida, Paula Rodrigues Fleck, Juliane Deise Zavaglia, Gabriela Oliveira Fernandes, Ingrid Rodrigues de David, Caroline Nespolo Santos, Amanda Paz de Almeida, Walquiria Aparecida Ferreira Porto, Victor Bertollo Gomes Scotta, Marcelo Comerlato Vieira, Gustavo Fioravanti Spilki, Fernando Rosado Stein, Renato T. Polese-Bonatto, Márcia Diagn Microbiol Infect Dis Article We aimed to describe the SARS-CoV-2 lineages circulating early pandemic among samples with S gene dropout and characterize the receptor-binding domain (RBD) of viral spike protein. Adults and children older than 2 months with signs and symptoms of COVID-19 were prospectively enrolled from May to October in Porto Alegre, Brazil. All participants performed RT-PCR assay, and samples with S gene dropout and cycle threshold < 30 were submitted to high-throughput sequencing (HTS). 484 out of 1,557 participants tested positive for SARS-CoV-2. The S gene dropout was detected in 7.4% (36/484) and a peak was observed in August. The B.1.1.28, B.1.91 and B.1.1.33 lineages were circulating in early pandemic. The RBD novel mutation (Y380Q) was found in one sample occurring simultaneously with C379W and V395A, and the B.1.91 lineage in the spike protein. The Y380Q and C379W may interfere with the binding of neutralizing antibodies (CR3022, EY6A, H014, S304). Elsevier Inc. 2022-04 2022-01-16 /pmc/articles/PMC8761118/ /pubmed/35219552 http://dx.doi.org/10.1016/j.diagmicrobio.2022.115636 Text en © 2022 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Sartor, Ivaine Tais Sauthier Varela, Fernanda Hammes Meireles, Mariana Rost Kern, Luciane Beatriz Azevedo, Thaís Raupp Giannini, Gabriela Luchiari Tumioto da Silva, Mariana Soares Demoliner, Meriane Gularte, Juliana Schons de Almeida, Paula Rodrigues Fleck, Juliane Deise Zavaglia, Gabriela Oliveira Fernandes, Ingrid Rodrigues de David, Caroline Nespolo Santos, Amanda Paz de Almeida, Walquiria Aparecida Ferreira Porto, Victor Bertollo Gomes Scotta, Marcelo Comerlato Vieira, Gustavo Fioravanti Spilki, Fernando Rosado Stein, Renato T. Polese-Bonatto, Márcia Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction |
title | Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction |
title_full | Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction |
title_fullStr | Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction |
title_full_unstemmed | Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction |
title_short | Y380Q novel mutation in receptor-binding domain of SARS-CoV-2 spike protein together with C379W interfere in the neutralizing antibodies interaction |
title_sort | y380q novel mutation in receptor-binding domain of sars-cov-2 spike protein together with c379w interfere in the neutralizing antibodies interaction |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761118/ https://www.ncbi.nlm.nih.gov/pubmed/35219552 http://dx.doi.org/10.1016/j.diagmicrobio.2022.115636 |
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