Protocol for the assessment of human T cell activation by real-time metabolic flux analysis

The elevation of glycolysis in autoreactive T cells is a key target for the prevention and treatment of T cell-related autoimmune diseases, such as type 1 diabetes (T1D). Here, we describe a simple and efficient protocol for isolating human peripheral blood mononuclear cells (PBMCs) and T cells, and...

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Detalles Bibliográficos
Autores principales: Kong, Byung Soo, Lee, Changhan, Cho, Young Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761778/
https://www.ncbi.nlm.nih.gov/pubmed/35072113
http://dx.doi.org/10.1016/j.xpro.2021.101084

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761778/
https://www.ncbi.nlm.nih.gov/pubmed/35072113
http://dx.doi.org/10.1016/j.xpro.2021.101084

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