Protocol for the assessment of human T cell activation by real-time metabolic flux analysis

The elevation of glycolysis in autoreactive T cells is a key target for the prevention and treatment of T cell-related autoimmune diseases, such as type 1 diabetes (T1D). Here, we describe a simple and efficient protocol for isolating human peripheral blood mononuclear cells (PBMCs) and T cells, and...

Descripción completa

Detalles Bibliográficos
Autores principales: Kong, Byung Soo, Lee, Changhan, Cho, Young Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761778/
https://www.ncbi.nlm.nih.gov/pubmed/35072113
http://dx.doi.org/10.1016/j.xpro.2021.101084
_version_ 1784633608591376384
author Kong, Byung Soo
Lee, Changhan
Cho, Young Min
author_facet Kong, Byung Soo
Lee, Changhan
Cho, Young Min
author_sort Kong, Byung Soo
collection PubMed
description The elevation of glycolysis in autoreactive T cells is a key target for the prevention and treatment of T cell-related autoimmune diseases, such as type 1 diabetes (T1D). Here, we describe a simple and efficient protocol for isolating human peripheral blood mononuclear cells (PBMCs) and T cells, and the subsequent assessment of T cell glycolysis using Seahorse analyzer. This protocol is useful to analyze different subsets of T cells and applicable to different autoimmune disease models (i.e., T1D, multiple sclerosis). For complete details on the use and execution of this profile, please refer to Kong et al. (2021).
format Online
Article
Text
id pubmed-8761778
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-87617782022-01-20 Protocol for the assessment of human T cell activation by real-time metabolic flux analysis Kong, Byung Soo Lee, Changhan Cho, Young Min STAR Protoc Protocol The elevation of glycolysis in autoreactive T cells is a key target for the prevention and treatment of T cell-related autoimmune diseases, such as type 1 diabetes (T1D). Here, we describe a simple and efficient protocol for isolating human peripheral blood mononuclear cells (PBMCs) and T cells, and the subsequent assessment of T cell glycolysis using Seahorse analyzer. This protocol is useful to analyze different subsets of T cells and applicable to different autoimmune disease models (i.e., T1D, multiple sclerosis). For complete details on the use and execution of this profile, please refer to Kong et al. (2021). Elsevier 2022-01-11 /pmc/articles/PMC8761778/ /pubmed/35072113 http://dx.doi.org/10.1016/j.xpro.2021.101084 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kong, Byung Soo
Lee, Changhan
Cho, Young Min
Protocol for the assessment of human T cell activation by real-time metabolic flux analysis
title Protocol for the assessment of human T cell activation by real-time metabolic flux analysis
title_full Protocol for the assessment of human T cell activation by real-time metabolic flux analysis
title_fullStr Protocol for the assessment of human T cell activation by real-time metabolic flux analysis
title_full_unstemmed Protocol for the assessment of human T cell activation by real-time metabolic flux analysis
title_short Protocol for the assessment of human T cell activation by real-time metabolic flux analysis
title_sort protocol for the assessment of human t cell activation by real-time metabolic flux analysis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761778/
https://www.ncbi.nlm.nih.gov/pubmed/35072113
http://dx.doi.org/10.1016/j.xpro.2021.101084
work_keys_str_mv AT kongbyungsoo protocolfortheassessmentofhumantcellactivationbyrealtimemetabolicfluxanalysis
AT leechanghan protocolfortheassessmentofhumantcellactivationbyrealtimemetabolicfluxanalysis
AT choyoungmin protocolfortheassessmentofhumantcellactivationbyrealtimemetabolicfluxanalysis

Ejemplares similares