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Protocol for the assessment of human T cell activation by real-time metabolic flux analysis

The elevation of glycolysis in autoreactive T cells is a key target for the prevention and treatment of T cell-related autoimmune diseases, such as type 1 diabetes (T1D). Here, we describe a simple and efficient protocol for isolating human peripheral blood mononuclear cells (PBMCs) and T cells, and...

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Detalles Bibliográficos
Autores principales:	Kong, Byung Soo, Lee, Changhan, Cho, Young Min
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8761778/ https://www.ncbi.nlm.nih.gov/pubmed/35072113 http://dx.doi.org/10.1016/j.xpro.2021.101084

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