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A New Tool for the Analysis of the Effect of Intracerebrally Injected Anti-Amyloid-β Compounds

BACKGROUND: A wide range of techniques has been developed over the past decades to characterize amyloid-β (Aβ) pathology in mice. Until now, no method has been established to quantify spatial changes in Aβ plaque deposition due to targeted delivery of substances using ALZET(®) pumps. OBJECTIVE: Deve...

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Autores principales: Upīte, Jolanta, Brüning, Thomas, Möhle, Luisa, Brackhan, Mirjam, Bascuñana, Pablo, Jansone, Baiba, Pahnke, Jens
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764605/
https://www.ncbi.nlm.nih.gov/pubmed/34719500
http://dx.doi.org/10.3233/JAD-215180
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author Upīte, Jolanta
Brüning, Thomas
Möhle, Luisa
Brackhan, Mirjam
Bascuñana, Pablo
Jansone, Baiba
Pahnke, Jens
author_facet Upīte, Jolanta
Brüning, Thomas
Möhle, Luisa
Brackhan, Mirjam
Bascuñana, Pablo
Jansone, Baiba
Pahnke, Jens
author_sort Upīte, Jolanta
collection PubMed
description BACKGROUND: A wide range of techniques has been developed over the past decades to characterize amyloid-β (Aβ) pathology in mice. Until now, no method has been established to quantify spatial changes in Aβ plaque deposition due to targeted delivery of substances using ALZET(®) pumps. OBJECTIVE: Development of a methodology to quantify the local distribution of Aβ plaques after intracerebral infusion of compounds. METHODS: We have developed a toolbox to quantify Aβ plaques in relation to intracerebral injection channels using Zeiss AxioVision(®) and Microsoft Excel(®) software. For the proof of concept, intracerebral stereotactic surgery was performed in 50-day-old APP-transgenic mice injected with PBS. At the age of 100 days, brains were collected for immunhistological analysis. RESULTS: The toolbox can be used to analyze and evaluate Aβ plaques (number, size, and coverage) in specific brain areas based on their location relative to the point of the injection or the injection channel. The tool provides classification of Aβ plaques in pre-defined distance groups using two different approaches. CONCLUSION: This new analytic toolbox facilitates the analysis of long-term continuous intracerebral experimental compound infusions using ALZET(®) pumps. This method generates reliable data for Aβ deposition characterization in relation to the distribution of experimental compounds.
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spelling pubmed-87646052022-02-04 A New Tool for the Analysis of the Effect of Intracerebrally Injected Anti-Amyloid-β Compounds Upīte, Jolanta Brüning, Thomas Möhle, Luisa Brackhan, Mirjam Bascuñana, Pablo Jansone, Baiba Pahnke, Jens J Alzheimers Dis Research Article BACKGROUND: A wide range of techniques has been developed over the past decades to characterize amyloid-β (Aβ) pathology in mice. Until now, no method has been established to quantify spatial changes in Aβ plaque deposition due to targeted delivery of substances using ALZET(®) pumps. OBJECTIVE: Development of a methodology to quantify the local distribution of Aβ plaques after intracerebral infusion of compounds. METHODS: We have developed a toolbox to quantify Aβ plaques in relation to intracerebral injection channels using Zeiss AxioVision(®) and Microsoft Excel(®) software. For the proof of concept, intracerebral stereotactic surgery was performed in 50-day-old APP-transgenic mice injected with PBS. At the age of 100 days, brains were collected for immunhistological analysis. RESULTS: The toolbox can be used to analyze and evaluate Aβ plaques (number, size, and coverage) in specific brain areas based on their location relative to the point of the injection or the injection channel. The tool provides classification of Aβ plaques in pre-defined distance groups using two different approaches. CONCLUSION: This new analytic toolbox facilitates the analysis of long-term continuous intracerebral experimental compound infusions using ALZET(®) pumps. This method generates reliable data for Aβ deposition characterization in relation to the distribution of experimental compounds. IOS Press 2021-12-07 /pmc/articles/PMC8764605/ /pubmed/34719500 http://dx.doi.org/10.3233/JAD-215180 Text en © 2021 – The authors. Published by IOS Press https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial (CC BY-NC 4.0) License (https://creativecommons.org/licenses/by-nc/4.0/) , which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Upīte, Jolanta
Brüning, Thomas
Möhle, Luisa
Brackhan, Mirjam
Bascuñana, Pablo
Jansone, Baiba
Pahnke, Jens
A New Tool for the Analysis of the Effect of Intracerebrally Injected Anti-Amyloid-β Compounds
title A New Tool for the Analysis of the Effect of Intracerebrally Injected Anti-Amyloid-β Compounds
title_full A New Tool for the Analysis of the Effect of Intracerebrally Injected Anti-Amyloid-β Compounds
title_fullStr A New Tool for the Analysis of the Effect of Intracerebrally Injected Anti-Amyloid-β Compounds
title_full_unstemmed A New Tool for the Analysis of the Effect of Intracerebrally Injected Anti-Amyloid-β Compounds
title_short A New Tool for the Analysis of the Effect of Intracerebrally Injected Anti-Amyloid-β Compounds
title_sort new tool for the analysis of the effect of intracerebrally injected anti-amyloid-β compounds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764605/
https://www.ncbi.nlm.nih.gov/pubmed/34719500
http://dx.doi.org/10.3233/JAD-215180
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