Imaging active site chemistry and protonation states: NMR crystallography of the tryptophan synthase α-aminoacrylate intermediate

NMR-assisted crystallography—the integrated application of solid-state NMR, X-ray crystallography, and first-principles computational chemistry—holds significant promise for mechanistic enzymology: by providing atomic-resolution characterization of stable intermediates in enzyme active sites, includ...

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Detalles Bibliográficos
Autores principales: Holmes, Jacob B., Liu, Viktoriia, Caulkins, Bethany G., Hilario, Eduardo, Ghosh, Rittik K., Drago, Victoria N., Young, Robert P., Romero, Jennifer A., Gill, Adam D., Bogie, Paul M., Paulino, Joana, Wang, Xiaoling, Riviere, Gwladys, Bosken, Yuliana K., Struppe, Jochem, Hassan, Alia, Guidoulianov, Jevgeni, Perrone, Barbara, Mentink-Vigier, Frederic, Chang, Chia-en A., Long, Joanna R., Hooley, Richard J., Mueser, Timothy C., Dunn, Michael F., Mueller, Leonard J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764694/
https://www.ncbi.nlm.nih.gov/pubmed/34996869
http://dx.doi.org/10.1073/pnas.2109235119

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764694/
https://www.ncbi.nlm.nih.gov/pubmed/34996869
http://dx.doi.org/10.1073/pnas.2109235119

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