Cargando…
Imaging active site chemistry and protonation states: NMR crystallography of the tryptophan synthase α-aminoacrylate intermediate
NMR-assisted crystallography—the integrated application of solid-state NMR, X-ray crystallography, and first-principles computational chemistry—holds significant promise for mechanistic enzymology: by providing atomic-resolution characterization of stable intermediates in enzyme active sites, includ...
Autores principales: | Holmes, Jacob B., Liu, Viktoriia, Caulkins, Bethany G., Hilario, Eduardo, Ghosh, Rittik K., Drago, Victoria N., Young, Robert P., Romero, Jennifer A., Gill, Adam D., Bogie, Paul M., Paulino, Joana, Wang, Xiaoling, Riviere, Gwladys, Bosken, Yuliana K., Struppe, Jochem, Hassan, Alia, Guidoulianov, Jevgeni, Perrone, Barbara, Mentink-Vigier, Frederic, Chang, Chia-en A., Long, Joanna R., Hooley, Richard J., Mueser, Timothy C., Dunn, Michael F., Mueller, Leonard J. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8764694/ https://www.ncbi.nlm.nih.gov/pubmed/34996869 http://dx.doi.org/10.1073/pnas.2109235119 |
Ejemplares similares
-
Solid-state (17)O NMR study of α-d-glucose: exploring new frontiers in isotopic labeling, sensitivity enhancement, and NMR crystallography
por: Shen, Jiahui, et al.
Publicado: (2022) -
Endogenously generated 2-aminoacrylate inhibits motility in Salmonella enterica
por: Borchert, Andrew J., et al.
Publicado: (2017) -
2-Aminoacrylate stress damages diverse PLP-dependent enzymes in vivo
por: Shen, Wangchen, et al.
Publicado: (2022) -
The Rid family member RutC of Escherichia coli is a 3-aminoacrylate deaminase
por: Buckner, Brandi A., et al.
Publicado: (2021) -
The Cysteine Desulfurase IscS Is a Significant Target of 2-Aminoacrylate Damage in Pseudomonas aeruginosa
por: Fulton, Ronnie L., et al.
Publicado: (2022)