Confounds of using the unc-58 selection marker highlights the importance of genotyping co-CRISPR genes

Multiple advances have been made to increase the efficiency of CRISPR/Cas9 editing using the model genetic organism Caenorhabditis elegans (C. elegans). Here we report on the use of co-CRISPR ‘marker’ genes: worms in which co-CRISPR events have occurred have overt, visible phenotypes which facilitat...

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Detalles Bibliográficos
Autores principales: Rawsthorne-Manning, Helena, Calahorro, Fernando, G. Izquierdo, Patricia, Tardy, Philippe, Boulin, Thomas, Holden-Dye, Lindy, O’Connor, Vincent, Dillon, James
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8765651/
https://www.ncbi.nlm.nih.gov/pubmed/35041685
http://dx.doi.org/10.1371/journal.pone.0253351

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8765651/
https://www.ncbi.nlm.nih.gov/pubmed/35041685
http://dx.doi.org/10.1371/journal.pone.0253351

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