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Modified Northern blot protocol for easy detection of mRNAs in total RNA using radiolabeled probes

BACKGROUND: Northern blotting is still used as a gold standard for validation of the data obtained from high-throughput whole transcriptome-based methods. However, its disadvantages of lower sensitivity, labor-intensive operation, and higher quality of RNA required limit its utilization in a routine...

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Detalles Bibliográficos
Autores principales: Yang, Tao, Zhang, Mingdi, Zhang, Nianhui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772191/
https://www.ncbi.nlm.nih.gov/pubmed/35057752
http://dx.doi.org/10.1186/s12864-021-08275-w