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Modified Northern blot protocol for easy detection of mRNAs in total RNA using radiolabeled probes
BACKGROUND: Northern blotting is still used as a gold standard for validation of the data obtained from high-throughput whole transcriptome-based methods. However, its disadvantages of lower sensitivity, labor-intensive operation, and higher quality of RNA required limit its utilization in a routine...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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BioMed Central
2022
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8772191/ https://www.ncbi.nlm.nih.gov/pubmed/35057752 http://dx.doi.org/10.1186/s12864-021-08275-w |