Additional Blue LED during Cultivation Induces Cold Tolerance in Tomato Fruit but Only to an Optimum

SIMPLE SUMMARY: LED lighting is increasingly applied to increase yield and quality of greenhouse produced crops, especially tomatoes. Tomatoes cannot be stored at cold temperatures due to chilling injury that manifests as quick quality deterioration during shelf life. The aim of this study is to investigate whether additional blue LED lighting can mitigate the negative effects of cold storage for ‘Foundation’ tomatoes. We applied three treatments, 0, 12 or 24% additional blue light during cultivation, and investigated quality attributes at harvest, after cold storage and subsequent shelf-life. We observed that red harvested tomatoes cultivated with 12% additional blue light acquired cold tolerance. Interestingly, these tomatoes were slightly less red colored at harvest and showed a faster loss of red color during cold storage. The measured red color is closely related to the lycopene concentration. We hypothesize that lycopene, a known antioxidant, present in 12% additional blue cultivated tomatoes mitigates chilling injury. Other antioxidants present in tomatoes were only affected by the ripeness at harvest and were therefore not involved in the acquired cold tolerance. The cultivation of tomatoes using additional blue LED is an attractive way to produce tomatoes that can withstand long transport at cold temperatures at the expense of a slightly less red tomato at the consumer. ABSTRACT: Tomato is a chilling-sensitive fruit. The aim of this study is to examine the role of preharvest blue LED lighting (BL) to induce cold tolerance in ‘Foundation’ tomatoes. Blue and red supplemental LED light was applied to achieve either 0, 12 or 24% additional BL (0B, 12B and 24B). Mature green (MG) or red (R) tomatoes were harvested and cold stored at 4 °C for 0, 5, 10, 15 and 20 d, and then stored for 20 d at 20 °C (shelf life). Chilling injury (CI) indices, color and firmness, hydrogen peroxide, malondialdehyde, ascorbic acid and catalase activity were characterized. At harvest, R tomatoes cultivated at 12B were firmer and showed less coloration compared to fruit of other treatments. These fruits also showed higher loss of red color during cold storage and lower CI symptoms during shelf-life. MG tomatoes cultivated at 12B showed delayed coloring (non-chilled) and decreased weight loss (long cold stored) during shelf life compared to fruit in the other treatments. No effects of light treatments, both for MG and R tomatoes, were observed for the selected antioxidant capacity indicators. Improved cold tolerance for R tomatoes cultivated at 12B points to lycopene having higher scavenging activity at lower concentrations to mitigate chilling injury.