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A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples

Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. Here we show that the widely used Bicinchoninic Acid (BCA) assay tends to underestimate protein concent...

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Detalles Bibliográficos
Autores principales: Helbing, Dario Lucas, Böhm, Leopold, Oraha, Nova, Stabenow, Leonie Karoline, Cui, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775029/
https://www.ncbi.nlm.nih.gov/pubmed/35049578
http://dx.doi.org/10.3390/gels8010043