Cargando…

A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples

Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. Here we show that the widely used Bicinchoninic Acid (BCA) assay tends to underestimate protein concent...

Descripción completa

Detalles Bibliográficos
Autores principales:	Helbing, Dario Lucas, Böhm, Leopold, Oraha, Nova, Stabenow, Leonie Karoline, Cui, Yan
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8775029/ https://www.ncbi.nlm.nih.gov/pubmed/35049578 http://dx.doi.org/10.3390/gels8010043

Ejemplares similares

Mouse sepsis models: don't forget ambient temperature!
por: Helbing, Dario Lucas, et al.
Publicado: (2022)

Immunoblotting and dot blotting
por: Stott, D.I.
Publicado: (1989)

Usefulness of the polymerase chain reaction dot-blot assay, used with Ziehl-Neelsen staining, for the rapid and convenient diagnosis of pulmonary tuberculosis in human immunodeficiency virus-seropositive and -seronegative individuals
por: Scherer, Luciene C., et al.
Publicado: (2011)

Safety and efficacy of ponceau 4R for cats, dogs and ornamental fish
por: Rychen, Guido, et al.
Publicado: (2018)

Rapid Screening of the Epidermal Growth Factor Receptor Phosphosignaling Pathway via Microplate-Based Dot Blot Assays
por: Cappione, Amedeo, et al.
Publicado: (2012)

Development of a PCR-based dot blot assay for the detection of fowl adenovirus
por: Hou, Lidan, et al.
Publicado: (2021)

Miniaturized fluorescent RNA dot blot method for rapid quantitation of gene expression
por: Yadetie, Fekadu, et al.
Publicado: (2004)

Development of an Indirect ELISA and Dot-Blot Assay for Serological Detection of Rice Tungro Disease
por: Henry Sum, Magdline Sia, et al.
Publicado: (2017)

Development of a quick dot blot assay for the titering of bovine ephemeral fever virus
por: Cheng, Li-Ting, et al.
Publicado: (2019)

Immobilization of DNA Aptamers on Polyester Cloth for Antigen Detection by Dot Blot Immunoenzymatic Assay (Aptablot)
por: Smiley, Sally, et al.
Publicado: (2013)

Detection of bovine viral diarrhea virus in serum from cattle by dot blot hybridization assay
por: Brock, K.V., et al.
Publicado: (1990)

Comparative performance of ELISA and dot blot assay for TSH‐receptor antibody detection in Graves’ disease
por: Zulkarnain, Zulkarnain, et al.
Publicado: (2022)

Theoretical study of Ponceau S oxidation using the electro-Fenton process under optimal operational conditions
por: Laftani, Yasmine, et al.
Publicado: (2023)

Latent Fingerprint Detection with SYPRO Rose Plus Protein Blot Stain
por: Bouldin, Kimberly K., et al.
Publicado: (2002)

Measurement of telomere DNA content by dot blot analysis
por: Kimura, Masayuki, et al.
Publicado: (2011)

Development of a sensitive PCR-dot blot assay to supplement serological tests for diagnosing Lyme disease
por: Shah, J. S., et al.
Publicado: (2017)

PCR colorimetric dot-blot assay and clinical pretest probability for diagnosis of Pulmonary Tuberculosis in Smear-Negative patients
por: Scherer, Luciene Cardoso, et al.
Publicado: (2007)

Evaluation of dot-blot test for serological diagnosis of bovine brucellosis
por: Bastos, Carla Resende, et al.
Publicado: (2018)

Rapid diagnosis of pulmonary tuberculosis and detection of drug resistance by combined simultaneous amplification testing and reverse dot blot
por: Chen, Yiwen, et al.
Publicado: (2018)

Serum HER2 Level Measured by Dot Blot: A Valid and Inexpensive Assay for Monitoring Breast Cancer Progression
por: Tan, Li-Duan, et al.
Publicado: (2011)

A reverse dot blot assay for the screening of twenty mutations in four genes associated with NSHL in a Chinese population
por: Li, Siping, et al.
Publicado: (2017)

Improved high-throughput quantification of luminescent microplate assays using a common Western-blot imaging system
por: Hawkins, Liam J., et al.
Publicado: (2017)

A Defined Methodology for Reliable Quantification of Western Blot Data
por: Taylor, Sean C., et al.
Publicado: (2013)

A Simple, Reproducible Procedure for Chemiluminescent Western Blot Quantification
por: Cañada-García, Daniel, et al.
Publicado: (2023)

Quantification of total and phosphorylated STAT3 by calibrated western blotting
por: Köhler, Nadine, et al.
Publicado: (2023)

Franconibacter sp., 1MS: A New Strain in Decolorization and Degradation of Azo Dyes Ponceau S Red and Methyl Orange
por: Baena-Baldiris, Dayana, et al.
Publicado: (2020)

A Serological Survey on Swine Brucellosis Using Standard Procedures, Dot Blot, and Western Blot in Finisher Pigs in Central-North Italy
por: Bertelloni, Fabrizio, et al.
Publicado: (2018)

Detection of Resistance to Fluoroquinolones and Second-Line Injectable Drugs Among Mycobacterium tuberculosis by a Reverse Dot Blot Hybridization Assay
por: Li, Guilian, et al.
Publicado: (2020)

Four Derivative Spectrophotometric Methods for the Simultaneous Determination of Carmoisine and Ponceau 4R in Drinks and Comparison with High Performance Liquid Chromatography
por: Turak, Fatma, et al.
Publicado: (2014)

Development of a recombinant protein based dot-blot for the diagnosis of canine leptospirosis
por: Conra, Neida, et al.
Publicado: (2014)

Simple and Sensitive Detection of HBsAg by Using a Quantum Dots Nanobeads Based Dot-Blot Immunoassay
por: Zhang, Pengfei, et al.
Publicado: (2014)

Evaluation of Dot-ELISA and enzyme-linked immuno-electrotransfer blot assays for detection of a urinary hydatid antigen in the diagnosis of cystic echinococcosis
por: Swarna, SR, et al.
Publicado: (2012)

A simple, inexpensive, robust and sensitive dot-blot assay for equal detection of the nonstructural-1 glycoprotein of all dengue virus serotypes
por: Falconar, Andrew KI, et al.
Publicado: (2013)

Clinical validation of a single-tube PCR and reverse dot blot assay for detection of common α-thalassaemia and β-thalassaemia in Chinese
por: Liang, Hong-Feng, et al.
Publicado: (2022)

Validation of western blot for Histoplasma capsulatum antibody detection assay
por: Almeida, Marcos de Abreu, et al.
Publicado: (2016)

Comparisons of ELISA and Western blot assays for detection of autophagy flux
por: Oh, Sung-hee, et al.
Publicado: (2017)

AuNP Coupled Rapid Flow-Through Dot-Blot Immuno-Assay for Enhanced Detection of SARS-CoV-2 Specific Nucleocapsid and Receptor Binding Domain IgG
por: Sil, Bijon Kumar, et al.
Publicado: (2021)

PCR-reverse blot hybridization assay in respiratory specimens for rapid detection and differentiation of mycobacteria in HIV-negative population
por: Zhang, Qing, et al.
Publicado: (2021)

Developing a routine lab test for absolute quantification of HER2 in FFPE breast cancer tissues using Quantitative Dot Blot (QDB) method
por: Yu, Guohua, et al.
Publicado: (2020)

Quantitative dot blot analysis (QDB), a versatile high throughput immunoblot method
por: Tian, Geng, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_ljesete06gntq58t622ovfmio9