Pipeline for 2-photon all-optical physiology in mouse: From viral titration and optical window implantation to binarization of calcium transients

2-photon all-optical physiology combines in vivo 2-photon calcium imaging and optogenetics, which enables both the read out and manipulation of neuronal microcircuits with single-cell resolution. Here, we describe a protocol for achieving optimized co-expression of calcium indicator and opsin. To en...

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Detalles Bibliográficos
Autores principales: Guimarães Backhaus, Roberta, Fu, Ting, Backhaus, Hendrik, Stroh, Albrecht
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776863/
https://www.ncbi.nlm.nih.gov/pubmed/35079708
http://dx.doi.org/10.1016/j.xpro.2021.101010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776863/
https://www.ncbi.nlm.nih.gov/pubmed/35079708
http://dx.doi.org/10.1016/j.xpro.2021.101010

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