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RTA and LANA Competitively Regulate let-7a/RBPJ Signal to Control KSHV Replication
The recombination signal binding protein for immunoglobulin kappa J region (RBPJ) has a dual effect on Kaposi’s sarcoma-associated herpesvirus (KSHV) replication. RBPJ interaction with replication and transcription activator (RTA) is essential for lytic replication, while the interaction with latenc...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777081/ https://www.ncbi.nlm.nih.gov/pubmed/35069510 http://dx.doi.org/10.3389/fmicb.2021.804215 |
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author | Di, Chunhong Zheng, Guoxia Zhang, Yunheng Tong, Enyu Ren, Yanli Hong, Yu Song, Yang Chen, Rong Tan, Xiaohua Yang, Lei |
author_facet | Di, Chunhong Zheng, Guoxia Zhang, Yunheng Tong, Enyu Ren, Yanli Hong, Yu Song, Yang Chen, Rong Tan, Xiaohua Yang, Lei |
author_sort | Di, Chunhong |
collection | PubMed |
description | The recombination signal binding protein for immunoglobulin kappa J region (RBPJ) has a dual effect on Kaposi’s sarcoma-associated herpesvirus (KSHV) replication. RBPJ interaction with replication and transcription activator (RTA) is essential for lytic replication, while the interaction with latency-associated nuclear antigen (LANA) facilitates latent infection. Furthermore, our previous study found that LANA decreased RBPJ through upregulating miRNA let-7a. However, it is unclear whether RTA regulates the expression of RBPJ. Here, we show RTA increases RBPJ by decreasing let-7a. During KSHV replication, the RBPJ expression level was positively correlated with the RTA expression level and negatively correlated with the LANA expression level. The let-7a expression level was inverse to RBPJ. Knockdown of RBPJ inhibited the self-activation of RTA promoter and LANA promoter and weakened LANA’s inhibition of RTA promoter. Collectively, these findings indicate that RTA and LANA compete for let-7a/RBPJ signal to control the KSHV replication. Regulating the RBPJ expression level by RTA and LANA plays an important role during KSHV replication. |
format | Online Article Text |
id | pubmed-8777081 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87770812022-01-22 RTA and LANA Competitively Regulate let-7a/RBPJ Signal to Control KSHV Replication Di, Chunhong Zheng, Guoxia Zhang, Yunheng Tong, Enyu Ren, Yanli Hong, Yu Song, Yang Chen, Rong Tan, Xiaohua Yang, Lei Front Microbiol Microbiology The recombination signal binding protein for immunoglobulin kappa J region (RBPJ) has a dual effect on Kaposi’s sarcoma-associated herpesvirus (KSHV) replication. RBPJ interaction with replication and transcription activator (RTA) is essential for lytic replication, while the interaction with latency-associated nuclear antigen (LANA) facilitates latent infection. Furthermore, our previous study found that LANA decreased RBPJ through upregulating miRNA let-7a. However, it is unclear whether RTA regulates the expression of RBPJ. Here, we show RTA increases RBPJ by decreasing let-7a. During KSHV replication, the RBPJ expression level was positively correlated with the RTA expression level and negatively correlated with the LANA expression level. The let-7a expression level was inverse to RBPJ. Knockdown of RBPJ inhibited the self-activation of RTA promoter and LANA promoter and weakened LANA’s inhibition of RTA promoter. Collectively, these findings indicate that RTA and LANA compete for let-7a/RBPJ signal to control the KSHV replication. Regulating the RBPJ expression level by RTA and LANA plays an important role during KSHV replication. Frontiers Media S.A. 2022-01-07 /pmc/articles/PMC8777081/ /pubmed/35069510 http://dx.doi.org/10.3389/fmicb.2021.804215 Text en Copyright © 2022 Di, Zheng, Zhang, Tong, Ren, Hong, Song, Chen, Tan and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Di, Chunhong Zheng, Guoxia Zhang, Yunheng Tong, Enyu Ren, Yanli Hong, Yu Song, Yang Chen, Rong Tan, Xiaohua Yang, Lei RTA and LANA Competitively Regulate let-7a/RBPJ Signal to Control KSHV Replication |
title | RTA and LANA Competitively Regulate let-7a/RBPJ Signal to Control KSHV Replication |
title_full | RTA and LANA Competitively Regulate let-7a/RBPJ Signal to Control KSHV Replication |
title_fullStr | RTA and LANA Competitively Regulate let-7a/RBPJ Signal to Control KSHV Replication |
title_full_unstemmed | RTA and LANA Competitively Regulate let-7a/RBPJ Signal to Control KSHV Replication |
title_short | RTA and LANA Competitively Regulate let-7a/RBPJ Signal to Control KSHV Replication |
title_sort | rta and lana competitively regulate let-7a/rbpj signal to control kshv replication |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777081/ https://www.ncbi.nlm.nih.gov/pubmed/35069510 http://dx.doi.org/10.3389/fmicb.2021.804215 |
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