Discrimination of the Activity of Low-Affinity Wild-Type and High-Affinity Mutant Recombinant BoNT/B by a SIMA Cell-Based Reporter Release Assay

Botulinum neurotoxin (BoNT) is used for the treatment of a number of ailments. The activity of the toxin that is isolated from bacterial cultures is frequently tested in the mouse lethality assay. Apart from the ethical concerns inherent to this assay, species-specific differences in the affinity fo...

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Detalles Bibliográficos
Autores principales: Neuschäfer-Rube, Frank, Pathe-Neuschäfer-Rube, Andrea, Püschel, Gerhard P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780439/
https://www.ncbi.nlm.nih.gov/pubmed/35051041
http://dx.doi.org/10.3390/toxins14010065

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8780439/
https://www.ncbi.nlm.nih.gov/pubmed/35051041
http://dx.doi.org/10.3390/toxins14010065

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