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Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors

Access to electron acceptors supports oxidized biomass synthesis and can be limiting for cancer cell proliferation, but how cancer cells overcome this limitation in tumors is incompletely understood. Nontransformed cells in tumors can help cancer cells overcome metabolic limitations, particularly in...

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Autores principales: Datta, Rupsa, Sivanand, Sharanya, Lau, Allison N., Florek, Logan V., Barbeau, Anna M., Wyckoff, Jeffrey, Skala, Melissa C., Vander Heiden, Matthew G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782446/
https://www.ncbi.nlm.nih.gov/pubmed/35061540
http://dx.doi.org/10.1126/sciadv.abg6383
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author Datta, Rupsa
Sivanand, Sharanya
Lau, Allison N.
Florek, Logan V.
Barbeau, Anna M.
Wyckoff, Jeffrey
Skala, Melissa C.
Vander Heiden, Matthew G.
author_facet Datta, Rupsa
Sivanand, Sharanya
Lau, Allison N.
Florek, Logan V.
Barbeau, Anna M.
Wyckoff, Jeffrey
Skala, Melissa C.
Vander Heiden, Matthew G.
author_sort Datta, Rupsa
collection PubMed
description Access to electron acceptors supports oxidized biomass synthesis and can be limiting for cancer cell proliferation, but how cancer cells overcome this limitation in tumors is incompletely understood. Nontransformed cells in tumors can help cancer cells overcome metabolic limitations, particularly in pancreatic cancer, where pancreatic stellate cells (PSCs) promote cancer cell proliferation and tumor growth. However, whether PSCs affect the redox state of cancer cells is not known. By taking advantage of the endogenous fluorescence properties of reduced nicotinamide adenine dinucleotide and oxidized flavin adenine dinucleotide cofactors we use optical imaging to assess the redox state of pancreatic cancer cells and PSCs and find that direct interactions between PSCs and cancer cells promote a more oxidized state in cancer cells. This suggests that metabolic interaction between cancer cells and PSCs is a mechanism to overcome the redox limitations of cell proliferation in pancreatic cancer.
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spelling pubmed-87824462022-02-07 Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors Datta, Rupsa Sivanand, Sharanya Lau, Allison N. Florek, Logan V. Barbeau, Anna M. Wyckoff, Jeffrey Skala, Melissa C. Vander Heiden, Matthew G. Sci Adv Biomedicine and Life Sciences Access to electron acceptors supports oxidized biomass synthesis and can be limiting for cancer cell proliferation, but how cancer cells overcome this limitation in tumors is incompletely understood. Nontransformed cells in tumors can help cancer cells overcome metabolic limitations, particularly in pancreatic cancer, where pancreatic stellate cells (PSCs) promote cancer cell proliferation and tumor growth. However, whether PSCs affect the redox state of cancer cells is not known. By taking advantage of the endogenous fluorescence properties of reduced nicotinamide adenine dinucleotide and oxidized flavin adenine dinucleotide cofactors we use optical imaging to assess the redox state of pancreatic cancer cells and PSCs and find that direct interactions between PSCs and cancer cells promote a more oxidized state in cancer cells. This suggests that metabolic interaction between cancer cells and PSCs is a mechanism to overcome the redox limitations of cell proliferation in pancreatic cancer. American Association for the Advancement of Science 2022-01-21 /pmc/articles/PMC8782446/ /pubmed/35061540 http://dx.doi.org/10.1126/sciadv.abg6383 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Datta, Rupsa
Sivanand, Sharanya
Lau, Allison N.
Florek, Logan V.
Barbeau, Anna M.
Wyckoff, Jeffrey
Skala, Melissa C.
Vander Heiden, Matthew G.
Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors
title Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors
title_full Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors
title_fullStr Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors
title_full_unstemmed Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors
title_short Interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors
title_sort interactions with stromal cells promote a more oxidized cancer cell redox state in pancreatic tumors
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8782446/
https://www.ncbi.nlm.nih.gov/pubmed/35061540
http://dx.doi.org/10.1126/sciadv.abg6383
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