Facile isolation of high-affinity nanobodies from synthetic libraries using CDR-swapping mutagenesis

The generation of high-affinity nanobodies for diverse biomedical applications typically requires immunization or affinity maturation. Here, we report a simple protocol using complementarity-determining region (CDR)-swapping mutagenesis to isolate high-affinity nanobodies from common framework libra...

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Detalles Bibliográficos
Autores principales: Zupancic, Jennifer M., Desai, Alec A., Tessier, Peter M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783142/
https://www.ncbi.nlm.nih.gov/pubmed/35098159
http://dx.doi.org/10.1016/j.xpro.2021.101101

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8783142/
https://www.ncbi.nlm.nih.gov/pubmed/35098159
http://dx.doi.org/10.1016/j.xpro.2021.101101

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