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Stem–loop formation drives RNA folding in mechanical unzipping experiments

Accurate knowledge of RNA hybridization is essential for understanding RNA structure and function. Here we mechanically unzip and rezip a 2-kbp RNA hairpin and derive the 10 nearest-neighbor base pair (NNBP) RNA free energies in sodium and magnesium with 0.1 kcal/mol precision using optical tweezers...

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Detalles Bibliográficos
Autores principales: Rissone, Paolo, Bizarro, Cristiano V., Ritort, Felix
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784153/
https://www.ncbi.nlm.nih.gov/pubmed/35022230
http://dx.doi.org/10.1073/pnas.2025575119