Investigation of sugar binding kinetics of the E. coli sugar/H(+) symporter XylE using solid-supported membrane-based electrophysiology

Bacterial transporters are difficult to study using conventional electrophysiology because of their low transport rates and the small size of bacterial cells. Here, we applied solid-supported membrane–based electrophysiology to derive kinetic parameters of sugar translocation by the Escherichia coli...

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Detalles Bibliográficos
Autores principales: Bazzone, Andre, Tesmer, Laura, Kurt, Derya, Kaback, H. Ronald, Fendler, Klaus, Madej, M. Gregor
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784342/
https://www.ncbi.nlm.nih.gov/pubmed/34929170
http://dx.doi.org/10.1016/j.jbc.2021.101505

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784342/
https://www.ncbi.nlm.nih.gov/pubmed/34929170
http://dx.doi.org/10.1016/j.jbc.2021.101505

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