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Investigation of sugar binding kinetics of the E. coli sugar/H(+) symporter XylE using solid-supported membrane-based electrophysiology
Bacterial transporters are difficult to study using conventional electrophysiology because of their low transport rates and the small size of bacterial cells. Here, we applied solid-supported membrane–based electrophysiology to derive kinetic parameters of sugar translocation by the Escherichia coli...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society for Biochemistry and Molecular Biology
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784342/ https://www.ncbi.nlm.nih.gov/pubmed/34929170 http://dx.doi.org/10.1016/j.jbc.2021.101505 |