A quantitative RT-PCR protocol to adapt and quantify RBM20-dependent exon splicing of targets at the human locus

Gene splicing is a fine-tuned process orchestrated by splice factors including RNA-binding motif 20 (RBM20), and their mutations are linked to the development of cardiac diseases. Here, we provide a step-by-step protocol to transfer RBM20-dependent splicing from rat to human. This protocol describes...

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Detalles Bibliográficos
Autores principales: Rebs, Sabine, Buchwald, Tjark Alexander, Streckfuss-Bömeke, Katrin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784397/
https://www.ncbi.nlm.nih.gov/pubmed/35106501
http://dx.doi.org/10.1016/j.xpro.2021.101117

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8784397/
https://www.ncbi.nlm.nih.gov/pubmed/35106501
http://dx.doi.org/10.1016/j.xpro.2021.101117

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