Cargando…
RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth
Adult skeletal muscle is a plastic tissue that can adapt its size to workload. Here, we show that RhoA within myofibers is needed for overload-induced hypertrophy by controlling satellite cell (SC) fusion to the growing myofibers without affecting protein synthesis. At the molecular level, we demons...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786647/ https://www.ncbi.nlm.nih.gov/pubmed/35106464 http://dx.doi.org/10.1016/j.isci.2021.103616 |
_version_ | 1784639158719873024 |
---|---|
author | Noviello, Chiara Kobon, Kassandra Delivry, Léa Guilbert, Thomas Britto, Florian Julienne, Francis Maire, Pascal Randrianarison-Huetz, Voahangy Sotiropoulos, Athanassia |
author_facet | Noviello, Chiara Kobon, Kassandra Delivry, Léa Guilbert, Thomas Britto, Florian Julienne, Francis Maire, Pascal Randrianarison-Huetz, Voahangy Sotiropoulos, Athanassia |
author_sort | Noviello, Chiara |
collection | PubMed |
description | Adult skeletal muscle is a plastic tissue that can adapt its size to workload. Here, we show that RhoA within myofibers is needed for overload-induced hypertrophy by controlling satellite cell (SC) fusion to the growing myofibers without affecting protein synthesis. At the molecular level, we demonstrate that RhoA controls in a cell autonomous manner Erk1/2 activation and the expressions of extracellular matrix (ECM) regulators such as Mmp9/Mmp13/Adam8 and macrophage chemo-attractants such as Ccl3/Cx3cl1. Their decreased expression in RhoA mutants is associated with ECM and fibrillar collagen disorganization and lower macrophage infiltration. Moreover, matrix metalloproteinases inhibition and macrophage depletion in controls phenocopied the altered growth of RhoA mutants while having no effect in mutants showing that their action is RhoA-dependent. These findings unravel the implication of RhoA within myofibers, in the building of a permissive microenvironment for muscle hypertrophic growth and for SC accretion through ECM remodeling and inflammatory cell recruitment. |
format | Online Article Text |
id | pubmed-8786647 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-87866472022-01-31 RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth Noviello, Chiara Kobon, Kassandra Delivry, Léa Guilbert, Thomas Britto, Florian Julienne, Francis Maire, Pascal Randrianarison-Huetz, Voahangy Sotiropoulos, Athanassia iScience Article Adult skeletal muscle is a plastic tissue that can adapt its size to workload. Here, we show that RhoA within myofibers is needed for overload-induced hypertrophy by controlling satellite cell (SC) fusion to the growing myofibers without affecting protein synthesis. At the molecular level, we demonstrate that RhoA controls in a cell autonomous manner Erk1/2 activation and the expressions of extracellular matrix (ECM) regulators such as Mmp9/Mmp13/Adam8 and macrophage chemo-attractants such as Ccl3/Cx3cl1. Their decreased expression in RhoA mutants is associated with ECM and fibrillar collagen disorganization and lower macrophage infiltration. Moreover, matrix metalloproteinases inhibition and macrophage depletion in controls phenocopied the altered growth of RhoA mutants while having no effect in mutants showing that their action is RhoA-dependent. These findings unravel the implication of RhoA within myofibers, in the building of a permissive microenvironment for muscle hypertrophic growth and for SC accretion through ECM remodeling and inflammatory cell recruitment. Elsevier 2021-12-11 /pmc/articles/PMC8786647/ /pubmed/35106464 http://dx.doi.org/10.1016/j.isci.2021.103616 Text en © 2021 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Noviello, Chiara Kobon, Kassandra Delivry, Léa Guilbert, Thomas Britto, Florian Julienne, Francis Maire, Pascal Randrianarison-Huetz, Voahangy Sotiropoulos, Athanassia RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth |
title | RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth |
title_full | RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth |
title_fullStr | RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth |
title_full_unstemmed | RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth |
title_short | RhoA within myofibers controls satellite cell microenvironment to allow hypertrophic growth |
title_sort | rhoa within myofibers controls satellite cell microenvironment to allow hypertrophic growth |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786647/ https://www.ncbi.nlm.nih.gov/pubmed/35106464 http://dx.doi.org/10.1016/j.isci.2021.103616 |
work_keys_str_mv | AT noviellochiara rhoawithinmyofiberscontrolssatellitecellmicroenvironmenttoallowhypertrophicgrowth AT kobonkassandra rhoawithinmyofiberscontrolssatellitecellmicroenvironmenttoallowhypertrophicgrowth AT delivrylea rhoawithinmyofiberscontrolssatellitecellmicroenvironmenttoallowhypertrophicgrowth AT guilbertthomas rhoawithinmyofiberscontrolssatellitecellmicroenvironmenttoallowhypertrophicgrowth AT brittoflorian rhoawithinmyofiberscontrolssatellitecellmicroenvironmenttoallowhypertrophicgrowth AT juliennefrancis rhoawithinmyofiberscontrolssatellitecellmicroenvironmenttoallowhypertrophicgrowth AT mairepascal rhoawithinmyofiberscontrolssatellitecellmicroenvironmenttoallowhypertrophicgrowth AT randrianarisonhuetzvoahangy rhoawithinmyofiberscontrolssatellitecellmicroenvironmenttoallowhypertrophicgrowth AT sotiropoulosathanassia rhoawithinmyofiberscontrolssatellitecellmicroenvironmenttoallowhypertrophicgrowth |