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A Real-Time PCR Assay for Detection of Low Pneumocystis jirovecii Levels

Here we report a new real-time PCR assay using SYBR Green which provides higher sensitivity for the specific detection of low levels of Pneumocystis jirovecii. To do so, two primer sets were designed, targeting the family of genes that code for the most abundant surface protein of Pneumocystis spp.,...

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Detalles Bibliográficos
Autores principales: Ruiz-Ruiz, Susana, Ponce, Carolina A., Pesantes, Nicole, Bustamante, Rebeca, Gatti, Gianna, San Martin, Viviana, Gutierrez, Mireya, Bórquez, Pamela, Vargas, Sergio L., Magne, Fabien, Calderón, Enrique J., Pérez-Brocal, Vicente, Moya, Andrés
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787145/
https://www.ncbi.nlm.nih.gov/pubmed/35087490
http://dx.doi.org/10.3389/fmicb.2021.787554