Enhancing biological signals and detection rates in single-cell RNA-seq experiments with cDNA library equalization

Considerable effort has been devoted to refining experimental protocols to reduce levels of technical variability and artifacts in single-cell RNA-sequencing data (scRNA-seq). We here present evidence that equalizing the concentration of cDNA libraries prior to pooling, a step not consistently perfo...

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Detalles Bibliográficos
Autores principales: Bacher, Rhonda, Chu, Li-Fang, Argus, Cara, Bolin, Jennifer M, Knight, Parker, Thomson, James A, Stewart, Ron, Kendziorski, Christina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789062/
https://www.ncbi.nlm.nih.gov/pubmed/34850101
http://dx.doi.org/10.1093/nar/gkab1071

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789062/
https://www.ncbi.nlm.nih.gov/pubmed/34850101
http://dx.doi.org/10.1093/nar/gkab1071

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