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Enhancing biological signals and detection rates in single-cell RNA-seq experiments with cDNA library equalization
Considerable effort has been devoted to refining experimental protocols to reduce levels of technical variability and artifacts in single-cell RNA-sequencing data (scRNA-seq). We here present evidence that equalizing the concentration of cDNA libraries prior to pooling, a step not consistently perfo...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Oxford University Press
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789062/ https://www.ncbi.nlm.nih.gov/pubmed/34850101 http://dx.doi.org/10.1093/nar/gkab1071 |